Document Type : Original Article
Authors
1
anatomy department,faculty of medicine,Minia university,Minia,Egypt
2
Department of Anatomy and Embryology, Faculty of Medicine, Fayoum University
3
Faculty of Medicine, Minia University
4
Department of Histology and Cell Biology, Faculty of Medicine, Menoufia University, Shebin Elkoum-Menoufia, Egypt
5
Department of Physiology, Faculty of Medicine, Al Azhar University, Assuit, Egypt
6
Department of Anatomy and Human Embryology, Kafr El Shiekh University, Faculty of Medicine, Kafrelsheikh Governorate, Egypt
Abstract
The anticancer medication methotrexate (MTX) is known to produce hepatotoxicity, a potentially hazardous side effect that restricts its therapeutic uses. Moringa leave extract (MLE) contains antioxidant and anti-inflammation ingredients; however, its significance in MTX-induced hepatic damage has not yet been examined. The current study aimed to investigate MLE's potential hepatoprotective efficacy against MTX-induced hepatotoxicity in rats.
Rats were treated with MLE orally two times a week for four weeks at al dosage of 300 mg/kg of total body weight, with or without an intraperitoneal injection of 0.5 mg/kg MTX injected twice a week for four weeks. Blood samples and livers tissues were obtained for biochemical and histopathological examination.
MTX impaired liver architecture and function, as evidenced by increased serum levels in liver enzymes ALT and AST. Furthermore, MTX generated oxidative stress, shown by raising MDA along with decreasing SOD, in addition it initiated inflammatory reaction through increased levels of Toll-like receptor 4 (TLR4), proinflammatory cytokines interleukin 1β (IL-1β) and Tumor Necrosis Factor Alpha (TNF-α) with increased hepatic tissues expression of caspase 3.
Interestingly, MLE with MTX significantly lowered all forms of oxidative stress and inflammation-related signaling with subsequent improving liver structure and function. As evidenced by inhibiting the activation of the caspase 3 pathway and down-regulating liver TLR4, IL-1β, and TNF-α. MLE has antioxidant, anti-inflammatory and anti-apoptotic effects that may diminish MTX-induced hepatotoxicity.
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